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</style><table class="infobox vevent"><tbody><tr><th colspan="2" class="infobox-above summary">PSF Lab</th></tr><tr><td colspan="2" class="infobox-image logo"><div class="infobox-caption">PSF Lab on Windows 7</div></td></tr><tr><th scope="row" class="infobox-label" style="white-space: nowrap;"><a href="Programmer" title="Programmer">Developer(s)</a></th><td class="infobox-data">Michael J. Nasse, Jörg C. Woehl</td></tr><tr style="display: none;"><td colspan="2" class="infobox-full-data"></td></tr><tr><th scope="row" class="infobox-label" style="white-space: nowrap;"><a href="Software_release_life_cycle" title="Software release life cycle">Stable release</a></th><td class="infobox-data"><div style="margin:0px;">3.0

/ September 24, 2010<span style="display:none"> (<span class="bday dtstart published updated">2010-09-24</span>)</span></div></td></tr><tr style="display:none"><td colspan="2">

</td></tr><tr><th scope="row" class="infobox-label" style="white-space: nowrap;"><a href="Operating_system" title="Operating system">Operating system</a></th><td class="infobox-data"><a href="Windows" class="mw-redirect" title="Windows">Windows</a>, <a href="MacOS" title="MacOS">macOS</a></td></tr><tr><th scope="row" class="infobox-label" style="white-space: nowrap;"><a href="Software_categories#Categorization_approaches" title="Software categories">Type</a></th><td class="infobox-data"><a href="List_of_computer_simulation_software" title="List of computer simulation software">Computer simulation software</a></td></tr><tr><th scope="row" class="infobox-label" style="white-space: nowrap;"><a href="Software_license" title="Software license">License</a></th><td class="infobox-data"><a href="Freeware" title="Freeware">Freeware</a> for academic and non-commercial use</td></tr><tr><th scope="row" class="infobox-label" style="white-space: nowrap;">Website</th><td class="infobox-data"><span class="url"><a rel="nofollow" class="external text" href="http://onemolecule.chem.uwm.edu/software">onemolecule<wbr>.chem<wbr>.uwm<wbr>.edu<wbr>/software</a></span></td></tr></tbody></table>

<p><b>PSF Lab</b> is a software program that allows the calculation of the illumination <a href="Point_spread_function" title="Point spread function">point spread function</a> (PSF) of a <a href="Confocal_microscope" class="mw-redirect" title="Confocal microscope">confocal microscope</a> under various imaging conditions. The calculation of the electric field vectors is based on a rigorous, vectorial model that takes <a href="Polarization_(waves)" title="Polarization (waves)">polarization</a> effects in the near-focus region and high <a href="Numerical_aperture" title="Numerical aperture">numerical aperture</a> <a href="Microscope_objective" class="mw-redirect" title="Microscope objective">microscope objectives</a> into account.<sup id="cite_ref-1" class="reference"><a href="#cite_note-1"><span class="cite-bracket">[</span>1<span class="cite-bracket">]</span></a></sup>

</p><p>The polarization of the input beam (assumed to be <a href="Collimated" class="mw-redirect" title="Collimated">collimated</a> and <a href="Monochromatic" class="mw-redirect" title="Monochromatic">monochromatic</a>) can be chosen freely (linear, circular, or elliptic). Furthermore, a constant or <a href="Gaussian_function" title="Gaussian function">Gaussian</a> shaped input beam intensity profile can be assumed. On its way from the <a href="Microscope_objective" class="mw-redirect" title="Microscope objective">objective</a> to the <a href="Focus_(optics)" title="Focus (optics)">focus</a>, the illumination light passes through up to three stratified optical layers, which allows the simulation of an <a href="Immersion_oil" class="mw-redirect" title="Immersion oil">immersion oil</a>/air (layer 1) objective that focusses light through a glass cover slip (layer 2) into the sample medium (layer 3). Each layer is characterized by its (constant) <a href="Refractive_index" title="Refractive index">refractive index</a> and thickness. PSF Lab can also simulate <a href="Microscope_objective" class="mw-redirect" title="Microscope objective">microscope objectives</a> that are corrected for certain refractive indices and cover slip thicknesses (design parameters). Thus, any deviations from the ideal imaging conditions for which the objective was designed for are properly taken into account.

</p><p>The following optical parameters can be selected:<sup id="cite_ref-2" class="reference"><a href="#cite_note-2"><span class="cite-bracket">[</span>2<span class="cite-bracket">]</span></a></sup>

</p>

<ul><li>Input beam

<ul><li><a href="Wavelength" title="Wavelength">Wavelength</a></li>

<li>Gaussian profile filling parameter (0 = constant profile)</li>

<li>Polarization (linear, circular, elliptic)</li></ul></li>

<li>Outputs

<ul><li>Individual field components</li>

<li>Squared field components</li>

<li>Intensity</li></ul></li>

<li>Microscope objective

<ul><li>Numerical aperture</li></ul></li>

<li>Optical media

<ul><li>Refractive index (design and actual)</li>

<li>Thickness (design and actual)</li>

<li>Depth (focus position within medium 3)</li></ul></li></ul>

<p>The program calculates only 2D section of the PSF, but several calculations can be stacked (with a third party program) to obtain the full 3D PSF. Calculations are organized in "sets", each with its own set of parameters. Loops can be set up such that PSF Lab calculates one or several sets, increasing the <a href="Image_resolution" title="Image resolution">resolution</a> of the calculated images in each new iteration. The resulting image is displayed in PSF Lab in linear or <a href="Logarithmic_scale" title="Logarithmic scale">logarithmic</a> color scale with user-selectable color map, and the intensity, individual field components, or squared field component distributions can be exported into various formats (data formats: .mat, .h5 (HDF5), .txt (ASCII); image formats: .fig, .ai, .bmp, .emf, .eps, .jpg, .pcx, .pdf, .png, .tif).

</p>

<ul><li><a href="Point_spread_function" title="Point spread function">Point spread function</a></li>

<li><a href="Optical_microscope" title="Optical microscope">Optical microscope</a></li>

<li><a href="Confocal_microscopy" title="Confocal microscopy">Confocal microscopy</a></li>

<li><a href="Confocal_laser_scanning_microscopy" class="mw-redirect" title="Confocal laser scanning microscopy">Confocal laser scanning microscopy</a></li></ul>

<div class="mw-heading mw-heading2"><h2 id="References">References</h2></div>

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</style><cite id="CITEREFNasse_M._J.,_Woehl_J._C.2010" class="citation journal cs1">Nasse M. J., Woehl J. C. (2010). <span class="id-lock-subscription" title="Paid subscription required"><a rel="nofollow" class="external text" href="http://www.opticsinfobase.org/josaa/viewmedia.cfm?uri=josaa-27-2-295&seq=0">"Realistic modeling of the illumination point spread function in confocal scanning optical microscopy"</a></span>. <i>J. Opt. Soc. Am. A</i>. <b>27</b> (2): <span class="nowrap">295–</span>302. <a href="Bibcode_(identifier)" class="mw-redirect" title="Bibcode (identifier)">Bibcode</a>:<a rel="nofollow" class="external text" href="https://ui.adsabs.harvard.edu/abs/2010JOSAA..27..295N">2010JOSAA..27..295N</a>. <a href="Doi_(identifier)" class="mw-redirect" title="Doi (identifier)">doi</a>:<a rel="nofollow" class="external text" href="https://doi.org/10.1364%2FJOSAA.27.000295">10.1364/JOSAA.27.000295</a>. <a href="PMID_(identifier)" class="mw-redirect" title="PMID (identifier)">PMID</a> <a rel="nofollow" class="external text" href="https://pubmed.ncbi.nlm.nih.gov/20126241">20126241</a>.</cite></span>

</li>

<li id="cite_note-2"><span class="mw-cite-backlink"><b><a href="#cite_ref-2">^</a></b></span> <span class="reference-text"><a rel="nofollow" class="external text" href="http://onemolecule.chem.uwm.edu/files/PSF%20Lab%20Getting%20Started.pdf">PSF Lab Getting Started Manual</a></span>

</li>

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<div class="mw-heading mw-heading2"><h2 id="External_links">External links</h2></div>

<ul><li><span class="official-website"><span class="url"><a rel="nofollow" class="external text" href="http://onemolecule.chem.uwm.edu/software">Official website</a></span></span></li>

<li><a rel="nofollow" class="external text" href="http://micro.magnet.fsu.edu/primer/digitalimaging/deconvolution/deconintro.html"><i>Molecular Expressions</i></a>, introduction to deconvolution using PSFs.</li></ul>

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